MONOCLONAL ANTI-MAT-TAG(TM), ANTIBODY P

Description_x000D_ General description_x000D_ Monoclonal Anti-MAT (mouse IgG2a isotype) is derived from the MAT1-87 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic MAT peptide HNHRHKHGGGC conjugated to KLH._x000D_ Monoclonal Anti-Mat-Tag™ antibody was used in one-step purification of histidine-tagged fusion proteins under high pressure and high flow conditions. It is used in the characterization of the molecular architecture of human caveolin-3 and interaction with the skeletal muscle ryanodine receptor._x000D_ Immunogen_x000D_ synthetic MAT peptide HNHRHKHGGGC._x000D_ Application_x000D_ The MAT tag is a seven amino acid peptide tag that binds to transition metals such as Nickel and Cobalt. This tag, which consists of the sequence HNHRHKH, allows the purification of MAT fusion proteins using immobilized metal affinity chromatography (IMAC) such as the highly selective HIS-Select® Nickel Affinity Gel. The MAT tag sequence can be incorporated into both prokaryotic and eukaryotic expression vectors._x000D_ Legal Information_x000D_ HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC_x000D_ MAT-Tag is a registered trademark of Sigma-Aldrich Co. LLC_x000D_ TagCFP is a trademark of Evrogen Co.