RIBONUCLEASE A SOLUTION MOLECULAR*BIOLOG

Description_x000D_ General description_x000D_ RNase A is an endoribonuclease that attacks at the 3’OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA._x000D_ Application_x000D_ Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays._x000D_ Suitable for:_x000D_ • RNase protection assays_x000D_ • Removal of unspecifically bound RNA_x000D_ • Analysis of RNA sequences_x000D_ • Hydrolysis of RNA contained in protein samples_x000D_ • Plasmid DNA purification_x000D_ Features and Benefits_x000D_ • RNase protection assays_x000D_ • Remove unspecifically bound RNA_x000D_ • Analysis of RNA sequences_x000D_ • Hydrolyze RNA contained in protein samples_x000D_ • Purification of DNA_x000D_ Components_x000D_ RNase A is supplied as a solution of 50% glycerol containing 10 mM Tris-HCl (pH 8.0)._x000D_ Unit Definition_x000D_ A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 10 ug/mL._x000D_ Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present._x000D_ Analysis Note_x000D_ Protein determined by E1%/280_x000D_ Other Notes_x000D_ Activators of RNase A include potassium and sodium salts. RNase A can be inhibited by alkylation of His12 or His119.